eBooks
Forensic Genetic Approaches for Identification of Human Skeletal Remains by
Unidentified human remains (UHRs) are associated with tragic events such as homicides, fires, terrorist attacks, natural disasters, war conflicts, genocide, airline crashes, human rights violations and forced disappearances under oppressive totalitarian regimes. In these situations, extensive damage to soft tissues often precludes the use of such biological samples in the identification process; in contrast, bone material is structurally resilient and is often the most viable (or the only available) types of sample for DNA testing. DNA recovered from bone can be very degraded and may exist in Low quantities due to the effects of human decomposition, environmental exposure, and the passage of time. The complexities of bone microstructure, its nonuniform patterns of diagenesis, and physical rigidity make skeletal remains one of the most challenging sample types for DNA testing. Specialized knowledge, equipment, and training are crucial to maximize success. Forensic Genetic Approaches for the Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies provides comprehensive coverage of established bone processing techniques and an overview of available DNA technologies for both contemporary casework and historical/archaeological investigations. The book focuses primarily on the use of DNA for direct identification or kinship analyses, but also highlights interdisciplinary approaches. Among the broad range of topics covered include: Detailed description of proper facilities design, contamination prevention measures, and specialized equipment needed for skeletal remains processing, Guidance on selection of optimal skeletal elements for DNA recovery, cleaning and preparation of bone samples for DNA testing, physical processing techniques (sectioning, pulverization), and DNA extraction methods, Overview of genetic markets available for identification of human skeletal remains, including autosomal short tandem repeats (STRs), Y-chromosome STRs, X-chromosome STRs, mitochondrial DNA (mtDNA), insertion/deletions (INDELS), ancestry-informative single nucleotide polymorphisms (SNPs), phenotype-informative SNPs, and identity-informative SNPs, Discussion of various platforms used to analyze DNA extracted from skeletal remains, including capillary electrophoresis (CE) instrumentation, massively parallel sequencing (MPS) systems, and field-deployable Rapid DNA equipment, Description of available databases and bioinformatic tools for genetic data analysis, including the Mass Fatality identification System (M-FISys), Overview of complementary disciplines often used in concert with genetic data to make positive identifications, including forensic anthropology, odontology, forensic art/sculpting (facial reconstructions), and genetic genealogy
ISBN: 9780128157664Publication Date: 2022-11-16
Genomic Applications in Pathology, 2d ed. by
The recent advances in genomics are continuing to reshape our approach to diagnostics, prognostics and therapeutics in oncologic and other disorders. A paradigm shift in pharmacogenomics and in the diagnosis of genetic inherited diseases and infectious diseases is unfolding as the result of implementation of next generation genomic technologies. With rapidly growing knowledge and applications driving this revolution, along with significant technologic and cost changes, genomic approaches are becoming the primary methods in many laboratories and for many diseases. As a result, a plethora of clinical genomic applications have been implemented in diagnostic pathology laboratories, and the applications and demands continue to evolve rapidly. This has created a tremendous need for a comprehensive resource on genomic applications in clinical and anatomic pathology. We believe that our current textbook provides such a resource to practicing molecular pathologists, hematopathologists and other subspecialized pathologists, general pathologists, pathology and other trainees, oncologists, geneticists and a growing spectrum of other clinicians. With periodic updates and a sufficiently rapid time from submission to publication, this textbook will be the resource of choice for many professionals and teaching programs. Its focus on genomics parallels the evolution of these technologies as primary methods in the clinical lab. The rapid evolution of genomics and its applications in medicine necessitates the (frequent) updating of this publication. This text will provide a state-of-the art review of the scientific principles underlying next generation genomic technologies and the required bioinformatics approaches to analyses of the daunting amount of data generated by current and emerging genomic technologies. Implementation roadmaps for various clinical assays such as single gene, gene panels, whole exome and whole genome assays will be discussed together with issues related to reporting and the pathologist's role in interpretation and clinical integration of genomic tests results. Genomic applications for site-specific solid tumors and hematologic neoplasms will be detailed. Genomic applications in pharmacogenomics, inherited genetic diseases and infectious diseases will also be discussed. The latest iteration of practice recommendations or guidelines in genomic testing put forth by stakeholder professional organizations such as the College of American Pathology and the Association for Molecular Pathology, will be discussed as well as regulatory issues and laboratory accreditation related to genomic testing. All chapters will be written by experts in their fields and will include the most up to date scientific and clinical information.
ISBN: 9783319968308
Stem Cell Laboratory Techniques by
Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study. Final sections cover useful and recent applications in stem cells, such as gene editing techniques and the preparation of stem cells for in vitro study, as well as stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics are discussed. Introduces readers to the stem cell culture and moves to handling techniques and versatile applications Includes coverage of gene editing techniques for stem cells and stem cells for in vitro study Presents stem cell lab design and equipment used in the lab
ISBN: 9780128237304Publication Date: 2023-05-27
eBooks
Stem Cell Therapy by
Stem cell therapy is a fast-growing field of medicine with remarkable prospects in a broad spectrum of diseases. Stem Cell Therapy: Practical Considerations addresses the biological properties of stem cells, mechanisms of action; as well as actual therapeutic decisions such as cell type, source, dose, manipulation, and route of injection. After discussing all this data, the book will illustrate how to travel through the idea from abstract question to laboratory experiment, animal experiment and then on to design a clinical trial throughout all its phases. Written for scientists and postgraduate students in the field of stem cell research and therapy. The authors will cover practical therapeutic issues they have long experienced in the field. Provides readers the basics and clinical practice of stem cell therapy Helps to debunk controversies regarding data fabrication in the field Guides the reader through the mechanisms of the regenerative function of stem cells; as well as the therapeutic decisions such as delivery route, delivery timing, cell doses, and follow-up
ISBN: 9780128215708Publication Date: 2022-11-13
Transpathology by
Transpathology: Molecular Imaging-Based Pathology is a multidisciplinary reference on molecular imaging and pathology. The book is intended for professionals in the fields of molecular imaging, nuclear medicine, radiology, and pathology as well as students and clinical residents. The book describes the importance of non-invasive diagnosis-based precision medicine and presents a detailed description of current transpathological approaches in different aspects essential for the future development of precision medicine. It is molecular imaging approach to experimental research and clinical practice which will drive the field forward and improve research outcomes.
ISBN: 9780323952231Publication Date: 2024-06-25
